摘要: The structural GDP/GTP cycle of Arf proteins is implemented by switch regions that change their conformations in response to the nature interactions. They include classical nucleotide-binding site, and a unique region located on opposite side protein. These communicate with each other couple activation Arfs GTP recruitment membranes. Variations this determine specificity differences Arf1 Arf6 signaling. role guanine nucleotide exchange factors (GEFs) as key players implementation nucleotide/membrane coupling also described. A particular emphasis given inhibition GEF-activated drug Brefeldin GEF point mutation, tools trap intermediate states reaction. Taken together, biochemical studies yield comprehensive model for one most remarkable found G proteins.
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