Development of a hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the determination of kinsenoside, an antihyperlipidemic candidate, in rat plasma and its application to pharmacokinetic studies.

摘要: Kinsenoside is a major bioactive constituent isolated from Anoectochilus formosanus and investigated as an antihyperlipidemic candidate. In this study, rapid, sensitive, reliable bioanalytical method was developed for the determination of kinsenoside in rat plasma using hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The sample pretreated with 1% acetic acid, followed by protein precipitation acetonitrile:methanol (70:30). Chromatographic separation performed on HILIC silica column (2.1mm×100mm, 3μm). mobile phases consisted 0.1% acid distilled water (solvent A) acetonitrile B). A gradient program used at flow rate 0.2mL/min. For spectrometric detection, multiple reaction monitoring mode used; MRM transitions were m/z 265.2→m/z 102.9 163.3→m/z 132.1 internal standard (IS) nicotine positive ionization mode. calibration curve constructed range 2-500ng/mL. intra- interday precision accuracy within 5%. HILIC-MS/MS specific, accurate, reproducible successfully applied pharmacokinetic study rats.