Coleta e preparação de pêlos de mamíferos para identificação em microscopia óptica

摘要: Hair microstructure is a useful tool to identify mammals and is applied in several disciplines such as forensic sciences, ecology, epidemiology, arqueology paleontology. The methods collect prepare mammal hairs identify them under the optical microscopy are many range from most cheap rapid expensive and laborious. present study aims test different collect, clean for cuticular and medullar patterns observation microscopy, compare these literature to describe detail new suggested method. To reference collection, were collected back of identified museum specimens with known collecting localities. Cuticular impressions obtained pressing hairs against thin nail varnish layer let dry 15 20 minutes on glass slides. In order observe medullar pattern, submitted diafanization comercial oxigenated water 30 volumes 80 minutes. Permanent glass slides mounted Entellan temporary ones glicerin. advantages of this method are the easy aquisition handling material practical execution, enabling its use field.