Agrobacterium tumefaciens -mediated transformation of an elite Australian barley cultivar with virus resistance and reporter genes
作者： Wang MingBo Wang MingBo , DC Abbott , NM Upadhyaya , JV Jacobsen , PM Waterhouse
DOI: 10.1071/PP00103
关键词:
摘要: Efficient transformation of barley cv. Schooner was achieved using Agrobacterium delivery, hygromycin or bialaphos selection and embryogenic callus. Using this system, transgenic plants were generated that contained either the green fluorescent protein gene, transgenes derived from yellow dwarf (BYDV) cereal (CYDV) viruses. Many these 1-3 transgene copies inherited in a simple Mendelian manner. Some containing BYDV and/or CYDV showed reduced virus symptoms rates viral replication when challenged with appropriate virus. The ability to transform is significant advance for Australian industry, as elite malting variety is, has last 15 years been, most widely grown eastern Australia.
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