Accumulation of glyceride-containing precursor of the outer membrane lipoprotein in the cytoplasmic membrane of Escherichia coli treated with globomycin.

摘要: The protein accumulated in the cell envelope of Escherichia coli treated with globomycin was identified as precursor outer membrane lipoprotein. prolipoprotein almost exclusively localized cytoplasmic membrane. could be immunoprecipitated antilipoprotein immunoglobulin and chased to lipoprotein both vivo vitro. Globomycin inhibited chase. contained glycerol fatty acid residues, whereas no free sulfhydryl group detected it. From these results, it is concluded that possesses a glyceride which covalently bound cysteine residue peptide does removal signal takes place after modification. inhibition bacterial growth increasing concentrations accompanied by gradual increase accumulation prolipoprotein. Furthermore, lipoprotein-negative mutant highly resistant globomycin. These results strongly indicate causes death cells.