Advanced Methods of Protein Crystallization.

摘要: This chapter provides a review of different advanced methods that help to increase the success rate crystallization project, by producing larger and higher quality single crystals for determination macromolecular structures crystallographic methods. For this purpose, is divided into three parts. The first part deals with fundamentals understanding process through strategies based on physical chemical approaches. second presents new approaches involved in more sophisticated not only growing protein but also controlling size orientation utilization electromagnetic fields other techniques. last section aspects: importance microgravity, use ligands stabilize proteins, microfluidics obtain crystals. All these will allow readers suitable crystalline samples high-resolution X-ray neutron crystallography.