Normal and pathological erythrocytes studied by atomic force microscopy.

摘要: Erythrocytes (red blood cells, RBCs) are the most common type of cells in vertebrates. Many diseases and dysfunctions directly affect their structure function. Employing atomic force microscope (AFM) physical, chemical, biological/physiological properties RBCs can be studied even under near-physiological conditions. In this chapter, we present application different AFM techniques to investigate compare normal pathological RBCs. We give a detailed description for nondestructive immobilization whole intact explain preparation isolated native RBC membranes. High-resolution imaging morphological details differences demonstrated with healthy systemic lupus erythematosus (SLE) erythrocytes revealing substructural changes due SLE. also technique simultaneous topography recognition imaging, which was used map distribution cystic fibrosis transmembrane conductance regulator sites on erythrocyte membranes fibrosis-positive