Bone Marrow and Adipose-Derived Mesenchymal Stem Cells Alleviate Methotrexate-Induced Pulmonary Fibrosis in Rat: Comparison with Dexamethasone.
作者： Ebtehal M. Fikry , Marwa M. Safar , Wedad A. Hasan , Hala M. Fawzy , Ezz-El-Din S. El-Denshary
DOI: 10.1002/JBT.21701
关键词:
摘要: Abstract The present study examined the therapeutic effects of bone marrow mesenchymal stem cells (BM-MSCs) and adipose-derived (AD-MSCs) in methotrexate (MTX)-induced pulmonary fibrosis rats as compared with dexamethasone (Dex). MTX (14 mg/kg, a single dose/week for 2 weeks, p.o.) induced lung marked by elevation relative weight, malondialdehyde, nitrite/nitrate, interleukin-4, transforming growth factor-β1, deposited collagen, well increased expression Bax along reduction reduced glutathione content superoxide dismutase activity. These deleterious were antagonized after treatment either BM-MSCs or AD-MSCs (2 × 10(6) cells/rat) weeks to even better extent than Dex (0.5 mg/kg/ 7 days, p.o.). In conclusion, BM-MSC possessed antioxidant, antiapoptotic, antifibrotic effects, which will probably introduce them remarkable candidates fibrosis.
sci-hub.se 参考文章(45)
Marshall I. Hertz, Linda S. Snyder, Cytotoxic drug-induced lung injury. Seminars in Respiratory Infections. ,vol. 3, pp. 217- 228 ,(1988)
Zhi-xin Liang, Ji-ping Sun, WANG Ping, TIAN Qing, YANG Zhen, Liang-an Chen, None, Bone marrow-derived mesenchymal stem cells protect rats from endotoxin-induced acute lung injury. Chinese Medical Journal. ,vol. 124, pp. 2715- ,(2011)
Yang CHENG, Jian PING, Lie-ming XU, Effects of curcumin on peroxisome proliferator-activated receptor γ expression and nuclear translocation/redistribution in culture-activated rat hepatic stellate cells Chinese Medical Journal. ,vol. 120, pp. 794- 801 ,(2007) , 10.1097/00029330-200705010-00011
Keyun Shi, Tieliang Ma, Lirong Duan, Zhixin Yu, Qin Zhu, Jinxu Zheng, Ruhua Chen, Jing Xie, Jianzhong Jiang, Ping Song, Chao Liu, Dexamethasone attenuates bleomycin-induced lung fibrosis in mice through TGF-β, Smad3 and JAK-STAT pathway. International Journal of Clinical and Experimental Medicine. ,vol. 7, pp. 2645- 2650 ,(2014)
Katrina M. Miranda, Michael G. Espey, David A. Wink, A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide. ,vol. 5, pp. 62- 71 ,(2001) , 10.1006/NIOX.2000.0319
Stan Gronthos, Andrew CW Zannettino, Shelley J Hay, Songtao Shi, Stephen E Graves, Angela Kortesidis, Paul J Simmons, Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. Journal of Cell Science. ,vol. 116, pp. 1827- 1835 ,(2003) , 10.1242/JCS.00369
Adel Alhadlaq, Jeremy J. Mao, Mesenchymal stem cells: isolation and therapeutics. Stem Cells and Development. ,vol. 13, pp. 436- 448 ,(2004) , 10.1089/SCD.2004.13.436
Dah-Ching Ding, Woei-Cherng Shyu, Shinn-Zong Lin, Mesenchymal Stem Cells Cell Transplantation. ,vol. 20, pp. 5- 14 ,(2011) , 10.3727/096368910X
Hye-Kyung Park, Kyu-Sup Cho, Hee-Young Park, Dong Hoon Shin, Yoon-Keun Kim, Jin Sup Jung, Soon Kew Park, Hwan Jung Roh, Adipose-Derived Stromal Cells Inhibit Allergic Airway Inflammation in Mice Stem Cells and Development. ,vol. 19, pp. 1811- 1818 ,(2010) , 10.1089/SCD.2009.0513
S. Herman, N. Zurgil, M. Deutsch, Low dose methotrexate induces apoptosis with reactive oxygen species involvement in T lymphocytic cell lines to a greater extent than in monocytic lines Inflammation Research. ,vol. 54, pp. 273- 280 ,(2005) , 10.1007/S00011-005-1355-8