Genetic diversity among Norwegian Vibrio parahaemolyticus.

作者: A.B. Ellingsen , H. Jørgensen , S. Wagley , M. Monshaugen , L.M. Rørvik

DOI: 10.1111/J.1365-2672.2008.03964.X

关键词:

摘要: Aims:  To examine the variability among environmental Vibrio parahaemolyticus (including trh+ isolates) from Norway, and to compare these clinical isolates imported foods. Methods Results:  A total of 246 V. were successfully digested with NotI, fragments separated by pulsed field gel electrophoresis (PFGE). The could be divided into 72 clusters 103 pulsotypes. Eleven clusters contained 4–31 isolates, within greatly varied respect origin. None /or tdh+ clustered trh−/tdh− isolates. included in study belonged two separate clusters. subset was serotyped, great serotype diversity observed parahaemolyticus. O3:K6 O3:KUT, identical or related a pandemic reference strain PFGE. Conclusions:  Environmental trh+) genetically diverse, but certain variants occurred throughout coastal environment, some persistent over time. Significance Impact Study:  Although persisted Norwegian no evidence indicated that indigenous have caused disease.

参考文章(38)
S. Marshall, C. G. Clark, G. Wang, M. Mulvey, M. T. Kelly, W. M. Johnson, Comparison of molecular methods for typing Vibrio parahaemolyticus. Journal of Clinical Microbiology. ,vol. 37, pp. 2473- 2478 ,(1999) , 10.1128/JCM.37.8.2473-2478.1999
Zahid Hayat Mahmud, Sucharit Basu Neogi, Afework Kassu, Bui Thi Mai Huong, Iqbal Kabir Jahid, Mohammad Sirajul Islam, Fusao Ota, Occurrence, seasonality and genetic diversity of Vibrio vulnificus in coastal seaweeds and water along the Kii Channel, Japan. FEMS Microbiology Ecology. ,vol. 64, pp. 209- 218 ,(2008) , 10.1111/J.1574-6941.2008.00460.X
J B Kaper, R K Campen, R J Seidler, M M Baldini, S Falkow, Cloning of the thermostable direct or Kanagawa phenomenon-associated hemolysin of Vibrio parahaemolyticus. Infection and Immunity. ,vol. 45, pp. 290- 292 ,(1984) , 10.1128/IAI.45.1.290-292.1984
M. Okura, R. Osawa, A. Iguchi, E. Arakawa, J. Terajima, H. Watanabe, Genotypic Analyses of Vibrio parahaemolyticus and Development of a Pandemic Group-Specific Multiplex PCR Assay Journal of Clinical Microbiology. ,vol. 41, pp. 4676- 4682 ,(2003) , 10.1128/JCM.41.10.4676-4682.2003
R.P. Maluping, C. Ravelo, C.R. Lavilla-Pitogo, K. Krovacek, J.L. Romalde, Molecular typing of Vibrio parahaemolyticus strains isolated from the Philippines by PCR-based methods. Journal of Applied Microbiology. ,vol. 99, pp. 383- 391 ,(2005) , 10.1111/J.1365-2672.2005.02571.X
Zahid Hayat Mahmud, Sucharit Basu Neogi, Afework Kassu, Takaomi Wada, M. Sirajul Islam, G. Balakrish Nair, Fusao Ota, Seaweeds as a reservoir for diverse Vibrio parahaemolyticus populations in Japan International Journal of Food Microbiology. ,vol. 118, pp. 92- 96 ,(2007) , 10.1016/J.IJFOODMICRO.2007.05.009
Zahid Hayat Mahmud, Afework Kassu, Alizadeh Mohammad, Masayuki Yamato, N.A. Bhuiyan, G. Balakrish Nair, Fusao Ota, Isolation and molecular characterization of toxigenic Vibrio parahaemolyticus from the Kii Channel, Japan. Microbiological Research. ,vol. 161, pp. 25- 37 ,(2006) , 10.1016/J.MICRES.2005.04.005