Induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes
作者： Mariya Petrova , Ely Zayova , Mariana Vlahova
DOI: 10.2478/S11535-013-0157-6
关键词:
摘要: The induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes mediated system was established. frequency genetic transformation varied from 4.8 to 12% depended on method infection. cefotaxime at concentration 200 mg/l proved suppress effectively the growth A. after co-cultivation. Among three tested nutrient media: Murashige and Skoog (MS), Gamborg’s (B5) Schenk Hildebrandt (SH), MS medium superior for high biomass production transformed compared other culture media. After culturing 40 days fresh weight clone T4 increased 7.6 fold over non-transformed roots. transfer rol A, B C genes into genome confirmed PCR analysis. Established techniques efficiently provided generated a large number — an excellent studying gene function could be used secondary metabolites synthesized
springer.com
cyberleninka.org
tpbin.com 参考文章(66)
David R. Murray, Advanced Methods in Plant Breeding and Biotechnology ,(1991)
D. R. Murray, E. M. Dommisse, A. J. Conner, M. C. Christey, J. E. Grant, Gene transfer to plants using Agrobacterium. Plant breeding and biotechnology. pp. 50- 73 ,(1991)
J. A. Teixeira da Silva, A. Giovannini, Tissue culture, cell culture and genetic transformation by wild type Agrobacterium rhizogenes in Mediterranean Helichrysum. Floriculture, ornamental and plant biotechnology. pp. 222- 226 ,(2006)
Dagmar Lange, Europe's medicinal and aromatic plants: their use, trade and conservation. Europe's medicinal and aromatic plants: their use, trade and conservation.. ,(1998)
Pamela J. Weathers, Barbara E. Wyslouzil, Yoojeong Kim, INVITED REVIEW: SECONDARY METABOLISM OF HAIRY ROOT CULTURES IN BIOREACTORS ,(2002)
Renate Müller, Brian Christensen, The Use of Agrobacterium rhizogenes and its rol-Genes for Quality Improvement in Ornamentals European Journal of Horticultural Science. ,vol. 74, pp. 275- 287 ,(2009)
Afsaneh SAMADI, Jirair CARAPETIAN, Reza HEIDARI, Morad JAFARI, Abdollah HASSANZADEH GORTTAPEH, Hairy Root Induction in Linum mucronatum ssp. mucronatum, an Anti-Tumor Lignans Producing Plant Notulae Botanicae Horti Agrobotanici Cluj-napoca. ,vol. 40, pp. 125- 131 ,(2012) , 10.15835/NBHA4017312
Margareta Welander, Li-Hua Zhu, RolGenes: Molecular Biology, Physiology, Morphology, Breeding Uses Plant Breeding Reviews. pp. 79- 103 ,(2010) , 10.1002/9780470650325.CH3
Gianfranco Gilardi, Cytochrome P450 biotechnology Biotechnology and Applied Biochemistry. ,vol. 60, pp. 1- 1 ,(2013) , 10.1002/BAB.1098
Sang Un Park, Jee Hee Park, Yong Kyoung Kim, Nam Il Park, Hui Xu, Sook Young Lee, Optimization of growth and pyranocoumarins production in hairy root culture of Angelica gigas Nakai Journal of Medicinal Plants Research. ,vol. 3, pp. 978- 981 ,(2009) , 10.5897/JMPR.9000952