In vitro models of lymphatic endothelial cells: cytokine receptor expression profiling and incorporation into organotypic culture models

摘要: Lymphatic endothelial cells (LECs) line the lymphatic vessels and lymph node sinuses. They function in balancing tissue interstitial fluid, trafficking of dendritic lymphocyte movement into out nodes, (lymph node-LECs), can modulate self-tolerance. LECs also are important for viral pathogenesis malignant cell migration. The discovery LEC-specific markers has enabled isolation culture during past decade, thereby increasing knowledge biology. Cytokines small proteins secreted by that have pleiotropic effects on survival, growth, functional activities. expression cytokine receptors LECs, if present, could provide insight potential functions LECs. current study defined receptor profile, especially IL6 family receptors, three LEC populations: human dermal microvascular (HMVEC-dLy), lung (HMVEC-LLy), telomerase reverse transcriptase transfected (hTERT-HDLEC), either with or without infection mimicry through use poly I:C treatment. Cytokine was examined at RNA, protein levels, as a strong reference further study. In vitro 3-dimensional (3D) is an substitute vivo experiments. It meaningful improvement to 2-dimensional modification environments better mimic situations. Using extracellular matrix extraction called MatrigelTM simplified 3D model substrate, we demonstrated five kinds (HMVEC-dLy, HMVEC-LLy, hTERT-HDLEC, ferret isolated macaque jejunal LECs) form network-like structure it (this process may be regarded modeling growth lymphangiogenesis). addition, this adapted organotypic containing preliminary build new powerful tool insights interactions between their environment. work represented thesis holds public health significances lying mainly enriching considering importance biology cancer development immunology.