Overexpression of p27KIP1 induced cell cycle arrest in G1 phase and subsequent apoptosis in HCC-9204 cell line

摘要: AIM: We have previously reported that inducible over-expression of Bak may prolong cell cycle in G1 phase and lead to apoptosis HCC-9204 cells. This study is investigate whether p27KIP1 plays an important role this process. METHODS: In order elucidate the exact function process, a zinc stable transfectant transie nt p27KIP1-GFP fusion were constructed. The effects on growth, arrest examined mock, control pMD vector, pMD-KIP1 transfected cells. RESULTS: transiently express gene. growth was reduced by 35% at 48 h induction with treatment as determined trypan blue exclusion assay. These differences remained same after 72 expression. caused 24 induction, 40% inc rease population. Prolonged expression line induced apoptotic death reflected TUNEL Fourty-eighth showed characteristic DNA ladder agarose gelelec trophoresis. CONCLUSION: induce through upregul ating subsequently protein can be expr essed expressing provides model assess function.