The role of interleukin-12 for mTOR regulation of memory CTLs

摘要: Title of Document: THE ROLE OF INTERLEUKIN-12 FOR MTOR REGULATION MEMORY CTLS. Karla Garcia, Master Science, 2014 Directed By: Associate Professor, Dr. Zhengguo Xiao, Department Animal and Avian Sciences A major goal vaccines is to induce functional immune memory, efforts improve the efficacy targeting memory CTLs have revealed an important immunoregulatory role rapamycin, a specific mTOR inhibitor. While inflammatory cytokines are critical for formation, it unknown if such as IL12 mediate rapamycin’s regulation during infection. Inhibition by rapamycin represses CTL expansion but enhances central vaccinia virus infection in mice. Without IL-12, effects on subsequent formation diminished, yet present compared not treated with rapamycin. In infected mice, directly IL-12 signaling WT upregulating receptor-β2 STAT4 phosphorylation. Furthermore, secondary rapamycin-regulated receptor knockouts impaired resultant abolished. This indicates that interplay between adjuvants should be considered vaccine design.