Detection of mRNAs for urokinase-type plasminogen activator, its receptor, and type 1 inhibitor in giant cell tumors of bone with in situ hybridization.

摘要: Although giant cell tumor of bone (GCT) is generally considered to be an uncommon benign neoplasm, it can pursue aggressive course with local recurrence and metastasis. Attempts predict the biological behavior GCT histopathological parameters, however, have not been successful. The urokinase-type plasminogen activation system has implicated in invasion metastasis abnormalities components this found several malignancies. In study we postulated that associated destruction present GCT. We therefore evaluated mRNA levels for activator (u-PA), receptor (u-PAR), inhibitor type 1 (PAI-1) by using Northern blot analysis situ hybridization four cases spindle-shaped mononuclear cells at 35th passage from a Our results showed tumors contained variable u-PA, u-PAR, PAI-1 mRNA, respectively, 2.3, 1.4, 3.2 kb size. were expressed both osteoclast-like cells; signal u-PA was more intense than multinuclear cells. Some spherical (macrophage-like cells) high comparison addition, passaged used gene expression during proliferation. level increases after adding 10% fetal calf serum quiescent induction maximal 16 hours remained 48 treatment. conclusion, even though are ultimately responsible resorption GCT, neoplastic may also involved degradation extracellular matrix invasive growth facilitating urokinase system. our observation upregulation stimulation indicated production linked growth.