作者: Ramakrishnan Melarkode , Shrikumar Suryanarayan , Kedarnath Nanjund Sastry , Lakshmi Prabha Varadarajalu , Akundi Venkata Sriram
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摘要: The present invention relates to a method of producing biologically active polypeptide having insulinotropic activity, the comprising steps of: (a) transforming genetically modified host cell that has protease gene knockout, with polynucleotide vector encoding polypeptide; and (b) growing transformed produce an N-terminal recognition site His-Gly Pichia pastoris STE13 polypeptide.