Proximity-dependent protein detection based on enzyme-assisted fluorescence signal amplification.
作者： Yuyu Tan , Qiuping Guo , Xiayu Zhao , Xiaohai Yang , Kemin Wang
DOI: 10.1016/J.BIOS.2013.08.001
关键词:
摘要: In this paper, we develop a sensitive fluorescence method for protein detection based on proximity extension and enzyme-assisted signal amplification. novel method, pairs of probes are designed, the recognition elements integrated into probes. Then proteins detected by transforming aptamer or antibody-protein binding signals DNA effect. addition, nick sites introduced to amplify detectable signal. As proof concept, human α-thrombin IgG demonstrated in study. The aptamers antibodies coupled as respectively. presence target protein, transformed effect, can be further amplified strand displacement. above mentioned strategies consequently bring limit (LOD) low 1 pM 6 IgG. Furthermore, might extended other changing
sci-hub.se 参考文章(50)
Eun Jeong Cho, Litao Yang, Matthew Levy, Andrew D. Ellington, Using a Deoxyribozyme Ligase and Rolling Circle Amplification To Detect a Non-nucleic Acid Analyte, ATP Journal of the American Chemical Society. ,vol. 127, pp. 2022- 2023 ,(2005) , 10.1021/JA043490U
M. Kamali-Moghaddam, C. Wadelius, U. Landegren, S. M. Gustafsdottir, J. Schlingemann, A. Rada-Iglesias, E. Schallmeiner, In vitro analysis of DNA-protein interactions by proximity ligation. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 104, pp. 3067- 3072 ,(2007) , 10.1073/PNAS.0611229104
Di Li, Bella Shlyahovsky, Johann Elbaz, Itamar Willner, Amplified Analysis of Low-Molecular-Weight Substrates or Proteins by the Self-Assembly of DNAzyme−Aptamer Conjugates Journal of the American Chemical Society. ,vol. 129, pp. 5804- 5805 ,(2007) , 10.1021/JA070180D
Amanda J. Haes, Lei Chang, William L. Klein, Richard P. Van Duyne, Detection of a biomarker for Alzheimer's disease from synthetic and clinical samples using a nanoscale optical biosensor. Journal of the American Chemical Society. ,vol. 127, pp. 2264- 2271 ,(2005) , 10.1021/JA044087Q
Ai-Xian Zheng, Jin-Ru Wang, Juan Li, Xiao-Rong Song, Guo-Nan Chen, Huang-Hao Yang, Nicking enzyme based homogeneous aptasensors for amplification detection of protein Chemical Communications. ,vol. 48, pp. 374- 376 ,(2012) , 10.1039/C1CC15853H
Guoxing Qin, Shulin Zhao, Yong Huang, Jing Jiang, Yi-Ming Liu, A sensitive gold nanoparticles sensing platform based on resonance energy transfer for chemiluminescence light on detection of biomolecules. Biosensors and Bioelectronics. ,vol. 46, pp. 119- 123 ,(2013) , 10.1016/J.BIOS.2013.02.011
Bhaskara V. Chikkaveeraiah, Ashwinkumar A. Bhirde, Nicole Y. Morgan, Henry S. Eden, Xiaoyuan Chen, Electrochemical Immunosensors for Detection of Cancer Protein Biomarkers ACS Nano. ,vol. 6, pp. 6546- 6561 ,(2012) , 10.1021/NN3023969
F. Barany, Genetic disease detection and DNA amplification using cloned thermostable ligase Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 189- 193 ,(1991) , 10.1073/PNAS.88.1.189
Juewen Liu, Zehui Cao, Yi Lu, Functional Nucleic Acid Sensors Chemical Reviews. ,vol. 109, pp. 1948- 1998 ,(2009) , 10.1021/CR030183I
Bella Shlyahovsky, Di Li, Yossi Weizmann, Roni Nowarski, Moshe Kotler, Itamar Willner, Spotlighting of cocaine by an autonomous aptamer-based machine. Journal of the American Chemical Society. ,vol. 129, pp. 3814- 3815 ,(2007) , 10.1021/JA069291N