Confining mycelial growth to porous microbeads: a novel technique to alter the morphology of non-newtonian mycelial cultures.

摘要: In an effort to alter the filamentous morphology of Penicillium chrysogenum cells, a technique was developed confine growth mycelia porous celite beads. The pore matrix these beads found be very effective for entrapping mycelial cells and spores. entrapped spores were used initiate fermentations in shake flask cultures. Significant increases final cell densities obtained confined cultures reaching up 60 g/L cells. This is nearly double concentration attainable free grown absence Cell loadings 0.55 g per bead later stages fermentations, specific oxygen uptake rates decrease with respect