Quantitative detection of β2-adrenergic agonists using fluorescence quenching by immunochromatographic assay

摘要: β2-Adrenergic agonists are banned in China and other areas the world. In this study, a novel method was developed to quantitatively detect β2-adrenergic agonists. The clorprenaline (CLP)–bovine serum albumin (BSA) conjugate is mixed with BSA–fluorescent microsphere (FM) complex sprayed on nitrocellulose membrane as test line; goat-anti-mouse antibody control line. If target molecule absent sample, colloidal gold-monoclonal will bind CLP–BSA coated line, gold quenches fluorescent microspheres, so that no signal develops indicating negative result. present sample at cutoff level or higher binds ELISA well. (Au-mAb) does not line indicates positive limit of detection (LOD) immunochromatographic assay strip 0.12 ng mL−1 when amount 0.8 μg time 15 min. could simultaneously five agonists, including clorprenaline, bambuterol, terbutaline, clenbuterol, salbutamol. When spiked swine urine samples (5.0 10.0 mL−1) were tested by immunoassay, recovery 39.00 ± 3.0 32.00 2.0, respectively.