Research on miR-126 in glioma targeted regulation of PTEN/PI3K/Akt and MDM2-p53 pathways.

摘要: Objective To study the correlations of micro ribonucleic acid (miR)-126 expression with pathogenesis and prognosis glioma, to screen potential biological targets for diagnosis, treatment glioma. Patients methods miR-126 in cancer tissues, normal brain U87MG cells astrocytes glioma patients was quantitatively analyzed via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). were transfected mimics or inhibitor, followed by verification qRT-PCR. The cell proliferation, apoptosis, migration invasion after transfection using methyl thiazolyl tetrazolium (MTT) assay, Annexin V/propidium iodide (PI) wound healing assay transwell respectively. levels proteins related phosphatase tensin homolog deleted on chromosome ten/phosphatidylinositol 3-kinase/protein kinase B (PTEN/PI3K/Akt) pathway double mouse minute 2 (MDM2)-p53 detected Western blotting. Moreover, prognostic analysis performed Kaplan-Meier method log-rank test. Results qRT-PCR showed that highly malignant tissues significantly lower than those cells, its level higher negative control group inhibitor. Analyses revealed up-regulation could remarkably inhibit in-vitro promote apoptosis vice versa. blotting manifested overexpression miR-126, PI3K, p-Akt MDM2 protein decreased compared group, but PTEN p53 expressions increased, Besides, according analysis, a low poorer. Conclusions is abnormally inhibits course through targeted regulation PTEN/PI3K/Akt MDM2-p53 pathways, which, therefore, can be used as new biomarker