Reverse signaling via CD30 ligand.

摘要: CD30 ligand (CD30L), a member of the TNF family, is type II membrane protein with C-terminal extracellular domain that homologous domains other family members. Also, like most members, N-terminal cytoplasmic CD30L conserved across species, but not between suggesting possible biological function. Motivated by this observation, we investigated potential for CD30L, when activated cross-linking, to directly transduce signal ligand-bearing cell. Cross-linking mAb or CD30-Fc fusion induced production IL-8 freshly isolated neutrophils. Further, both cross-linking mechanisms produced rapid oxidative burst. Indirect effects through were ruled out, since CD30, expressed on Expression can be in peripheral blood T cells CD3 component TCR. Peripheral exposed suboptimal concentrations anti-CD3 increased metabolic activity, proliferated, and IL-6 response CD30L. These results indicate cross-linked This "reverse signaling" via taken together previously published data concerning ligands strongly suggest that, as rule, members their cognate receptors bidirectionally, blurring distinction receptor.