Highly sensitive naphthalimide-based fluorescence polarization probe for detecting cancer cells.

摘要: Fluorescence polarization (FP)-based signal is a self-referencing fluorescence signal, and it less dependent on dye concentration environmental interferences, which makes FP measurement an attractive alternative sensing technology to intensity-based detection. However, most of the probes were constructed by introducing fluorescein, rhodamine, cyanine dyes, have relatively shorter excited-state lifetimes compared with BODIPY naphthalimide dyes. Herein, first based probe (BIO) was designed synthesized for selective direct detection cancer cells. The longer high photostability BIO more sensitive accuracy in quantitative determination HeLa cells homogeneous solution without cell lysis further separation steps. limit about 85 mL–1, linear range from 2.5 × 102 mL–1 t...