The enzymatic synthesis of N-acetyl-L-aspartic acid by subcellular preparations of rat brain.

摘要: Abstract The acetyl coenzyme A-l-aspartate N-acetyltransferase activity of a previously reported preparation obtained from the supernatant phase rat brain homogenates by ammonium sulfate fractionation was reinvestigated and substantiated. enzyme located in particles, probably microsomes, dispersed fluid. A homogenate system with comparatively high N-acetylaspartate synthesizing capacity presence acetyl-CoA l-aspartate is described. this recovered association particulate structures. Acetone-dried powders crude mitochondrial fraction microsomal had specific activities approximating 40 20% that respective fresh tissue constituents.