Design of the solid phase for protein arrays and use of semiconductor nanoparticles as reporters in immunoassays

摘要: Publisher Summary A fundamental problem encountered while using polymer solid phase in immunoassays is non-specific protein adsorption from blood or serum. Grafting of functionalized poly(ethylene glycol) (PEG) to microparticles and planar quartz surface induces resistance. In this chapter, we review several methods PEG modification micro- nanoparticles discuss the behavior non-ionic polymers at solid/liquid interfaces. We have demonstrated that coated with can be used as a immunoassays, both for immobilization antibodies presentation various antigens. also devised convenient method grafting monolayers surfaces. Here, photophysical properties available fluorescent labels potentially useful reporters cell analyses, recent advances development luminescent semiconductor quantum dots (Qdots). comparison organic dyes proteins, Qdots represent new class unique spectral properties. The fluorescence intensity single nanocrystal species (Qdot-655) were investigated context panel containing conventional fluorophores.