Surface plasmon resonance analysis to evaluate the importance of heparin sulfate groups' binding with human aFGF and bFGF

摘要: Human acidic and basic fibroblast growth factors (aFGF bFGF) are classic well characterized members of the heparin-binding factor family. Heparin is generally thought to play an extremely important role in regulating aFGF bFGF bioactivities through its strong binding with them. In order unravel mechanism interactions between heparin FGFs, evaluate importance sulfate groups' surface plasmon resonance analyses were performed using IAsys Cuvettes System. regioselectively desulfated derivatives immobilized on cuvettes. solutions different concentrations pipetted into cuvettes progress interaction was monitored real-time by Windows-based software, yielding kinetic equilibrium constants for these interactions. addition, reduce delicate difference among cuvettes, inhibition mixtures FGFs native modified heparins also done. The data from two methods similar, indicating that all groups at 2-O, 6-O N- required aFGF; their contribution 6-O-sulfate group. contrast, definite group most apparent, while other appeared be necessary 2-O N-sulfate These established here can used analysing effect human FGF or proteins.