Tyrosine Phosphorylation Regulates Rapid Endocytosis in Adrenal Chromaffin Cells

摘要: Secretion of neurotransmitter at the synapse and in secretory cells depends on availability vesicles for exocytosis. Rapid endocytosis is responsible initiating local vesicle recycling essential during sustained neurotransmission. Although exocytosis triggered by Ca2+ influx modulated serine/threonine kinases, relatively little known about regulation rapid endocytosis. Our data suggest that controlled tyrosine phosphorylation. Treatment bovine adrenal chromaffin with tyrphostin 23, a protein kinase inhibitor, dramatically slowed time course In contrast, there was no effect either amount or rate Application orthovanadate, Zn2+, poly(Glu, Tyr) (4:1), each which phosphatase reversed 23 Thus endocytosis, like exocytosis, subject to intracellular signaling pathways.