Comparative genomics of selected species of gram-negative bacteria.

作者: Chuan-Peng Ren

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摘要: Investigation of genomic diversity can provide insight into the evolutionary history bacterial species. However, complete genome sequencing is not yet practical for large strain collections at beginning this project. In project PCR-based methods to investigate non-sequenced strains were successfully developed. \(Escherichia\) \(coli\), distribution two Type III secretion system, ETT2 (\(E.\) \(coli\) Three Secretion 2) and Flag-2 Flagellar system 2), surveyed among a collection 79 strains. Remnants both clusters found be present in most strains, suggesting that have long within \(E.\) \(coli\). The also developed application as part projects. They used explore co-linear variable regions between \(Campylobacter\) \(jejuni\) M1 sequenced \(C.\) 11168. was assembled thirty-four contigs relative 11168, size position insertions/deletions characterised. Similar facilitate finishing \(Francisella\) \(tularensis\) FSC198, using sequence information from Schu S4. completed FSC198 showed it almost identical genomes differ only 11 loci, eight SNPs (single nucleotide polymorphisms) 3 VNTRs (variable number tandem repeats). This surprising finding suggested European isolate may derived US laboratory Two virulence factors, IglA IglB, pathogenicity island further investigated interact protein level. These proteins are possibly involved VI secretion, represent potential vaccine candidates.

参考文章(223)
Darina Guryčová, First isolation of Francisella tularensis subsp. tularensis in Europe European Journal of Epidemiology. ,vol. 14, pp. 797- 802 ,(1998) , 10.1023/A:1007537405242
Joerg Jores, Leonid Rumer, Lothar H. Wieler, IMPACT OF THE LOCUS OF ENTEROCYTE EFFACEMENT PATHOGENICITY ISLAND ON THE EVOLUTION OF PATHOGENIC ESCHERICHIA COLI International Journal of Medical Microbiology. ,vol. 294, pp. 103- 113 ,(2004) , 10.1016/J.IJMM.2004.06.024
Michael S. Donnenberg, Escherichia coli : virulence mechanisms of a versatile pathogen Academic Press. ,(2002)
Steve Rozen, Helen Skaletsky, Primer3 on the WWW for general users and for biologist programmers. Methods of Molecular Biology. ,vol. 132, pp. 365- 386 ,(2000) , 10.1385/1-59259-192-2:365
Christian Baron, David O´Callaghan, Erich Lanka, Bacterial secrets of secretion: EuroConference on the biology of type IV secretion processes Molecular Microbiology. ,vol. 43, pp. 1359- 1365 ,(2002) , 10.1046/J.1365-2958.2002.02816.X
Claire M Fraser, Jonathan Eisen, Robert D Fleischmann, Karen A Ketchum, Scott Peterson, Comparative genomics and understanding of microbial biology. Emerging Infectious Diseases. ,vol. 6, pp. 505- 512 ,(2000) , 10.3201/EID0605.000510
F. Sanger, G. M. Air, B. G. Barrell, N. L. Brown, A. R. Coulson, J. C. Fiddes, C. A. Hutchison, P. M. Slocombe, M. Smith, Nucleotide sequence of bacteriophage φX174 DNA Nature. ,vol. 265, pp. 687- 695 ,(1977) , 10.1038/265687A0
Jeffrey H Miller, Experiments in molecular genetics ,(1972)