作者: Chuan-Peng Ren
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摘要: Investigation of genomic diversity can provide insight into the evolutionary history bacterial species. However, complete genome sequencing is not yet practical for large strain collections at beginning this project. In project PCR-based methods to investigate non-sequenced strains were successfully developed. \(Escherichia\) \(coli\), distribution two Type III secretion system, ETT2 (\(E.\) \(coli\) Three Secretion 2) and Flag-2 Flagellar system 2), surveyed among a collection 79 strains. Remnants both clusters found be present in most strains, suggesting that have long within \(E.\) \(coli\). The also developed application as part projects. They used explore co-linear variable regions between \(Campylobacter\) \(jejuni\) M1 sequenced \(C.\) 11168. was assembled thirty-four contigs relative 11168, size position insertions/deletions characterised. Similar facilitate finishing \(Francisella\) \(tularensis\) FSC198, using sequence information from Schu S4. completed FSC198 showed it almost identical genomes differ only 11 loci, eight SNPs (single nucleotide polymorphisms) 3 VNTRs (variable number tandem repeats). This surprising finding suggested European isolate may derived US laboratory Two virulence factors, IglA IglB, pathogenicity island further investigated interact protein level. These proteins are possibly involved VI secretion, represent potential vaccine candidates.