Chitosan scaffolds containing hyaluronic acid for cartilage tissue engineering.
作者： Clara R. Correia , Liliana S. Moreira-Teixeira , Lorenzo Moroni , Rui L. Reis , Clemens A. van Blitterswijk
DOI: 10.1089/TEN.TEC.2010.0467
关键词:
摘要: Scaffolds derived from natural polysaccharides are very promising in tissue engineering applications and regenerative medicine, as they resemble glycosaminoglycans the extracellular matrix (ECM). In this study, we have prepared freeze-dried composite scaffolds of chitosan (CHT) hyaluronic acid (HA) different weight ratios containing either no HA (control) or 1%, 5%, 10% HA. We hypothesized that could enhance structural biological properties CHT scaffolds. To test hypothesis, physicochemical CHT/HA were evaluated. Scanning electron microscopy micrographs, mechanical properties, swelling tests, enzymatic degradation, Fourier transform infrared (FTIR) chemical maps performed. ability to support chondrocyte adhesion proliferation, live–dead MTT assays Results showed noncytotoxic promote cell adhesion. ECM formation was further evaluated with safranin-O alcian blue staining methods, glycosaminoglycan DNA quantifications The incorporation enhanced cartilage production. CHT/5HA had a better pore network configuration exhibited formation. On basis our results, believe matrixes potential use repair.
core.ac.uk
utwente.nl
narcis.nl
researchgate.net 参考文章(57)
I Prasadam, Y Xiao, A Oloyede, N Chakravorty, R Crawford, Saso Ivanovski, MicroRNAs potentially regulate TGFb/BMP & Wnt/Ca2+ signaling pathways on modified titanium implant surfaces leading to improved osteogenicity Journal of Tissue Engineering and Regenerative Medicine. ,vol. 6, pp. 229- 229 ,(2012) , 10.1002/TERM.1586
Nandana Bhardwaj, Subhas C. Kundu, Chondrogenic differentiation of rat MSCs on porous scaffolds of silk fibroin/chitosan blends. Biomaterials. ,vol. 33, pp. 2848- 2857 ,(2012) , 10.1016/J.BIOMATERIALS.2011.12.028
R Nordtveit, Degradation of partially N-acetylated chitosans with hen egg white and human lysozyme Carbohydrate Polymers. ,vol. 29, pp. 163- 167 ,(1996) , 10.1016/0144-8617(96)00003-3
KLAUS VON DER MARK, VERENA GAUSS, HELGA VON DER MARK, PETER MÜLLER, Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture Nature. ,vol. 267, pp. 531- 532 ,(1977) , 10.1038/267531A0
Y JI, K GHOSH, X SHU, B LI, J SOKOLOV, G PRESTWICH, R CLARK, M RAFAILOVICH, Electrospun three-dimensional hyaluronic acid nanofibrous scaffolds Biomaterials. ,vol. 27, pp. 3782- 3792 ,(2006) , 10.1016/J.BIOMATERIALS.2006.02.037
S.H Pangburn, P.V Trescony, J Heller, Lysozyme degradation of partially deacetylated chitin, its films and hydrogels. Biomaterials. ,vol. 3, pp. 105- 108 ,(1982) , 10.1016/0142-9612(82)90043-6
H TAN, J WU, L LAO, C GAO, Gelatin/chitosan/hyaluronan scaffold integrated with PLGA microspheres for cartilage tissue engineering. Acta Biomaterialia. ,vol. 5, pp. 328- 337 ,(2009) , 10.1016/J.ACTBIO.2008.07.030
Amos Matsiko, Tanya J. Levingstone, Fergal J. O’Brien, John P. Gleeson, Addition of hyaluronic acid improves cellular infiltration and promotes early-stage chondrogenesis in a collagen-based scaffold for cartilage tissue engineering. Journal of The Mechanical Behavior of Biomedical Materials. ,vol. 11, pp. 41- 52 ,(2012) , 10.1016/J.JMBBM.2011.11.012
J.J.A. Barry, H.S. Gidda, C.A. Scotchford, S.M. Howdle, Porous methacrylate scaffolds: supercritical fluid fabrication and in vitro chondrocyte responses Biomaterials. ,vol. 25, pp. 3559- 3568 ,(2004) , 10.1016/J.BIOMATERIALS.2003.10.023
Kenji Tomihata, Yoshito Ikada, In vitro and in vivo degradation of films of chitin and its deacetylated derivatives Biomaterials. ,vol. 18, pp. 567- 575 ,(1997) , 10.1016/S0142-9612(96)00167-6