Virus-encoded cyclin.

摘要: Herpesvirus saimiri contains an open reading frame called eclf2 with homology to the cellular type D cyclins. We now show that gene product is a novel virus-encoded cyclin (v-cyclin). The protein encoded by v-cyclin of this oncogenic herpesvirus was found have apparent molecular size 29 kDa in transformed cells. v-Cyclin be associated cdk6, cyclin-dependent kinase known interact cdk6/v-cyclin complexes strongly phosphorylated Rb fusion and histone H1 as substrates vitro. Mutational analyses showed highly conserved amino acids box were important for association cdk6 activation activity. Thus, resembles cyclins primary sequence, its ability activate activity, presence functional sequences. exhibited selective preference over other kinases high level activation. properties suggest likely role transformation T-lymphotropic herpesvirus.