Two‐Dimensional protein map of Saccharomyces cerevisiae: Construction of a gene–protein index
作者： Helian Boucherie , Christelle Monribot , Michel Perrot , Genevieve Dujardin , Michele Kermorgant
关键词:
摘要: This publication marks the beginning of construction a gene-protein index that relates proteins which are resolved on two-dimensional protein map Saccharomyces cerevisiae with their corresponding genes. We report identification 36 novel polypeptide spots yeast map. They correspond to products 26 Together previously identified, this raises 41 number genes whose have been identified The here concerned four major areas cellular physiology: carbon metabolism, heat shock, amino acid biosynthesis and purine biosynthesis. Given molecular weight isoelectric point proteins, codon-usage bias genes, it can be estimated 25 35% all soluble detectable under labelling running gel conditions used in study.
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