Analysis of neuroactive amines in fermented beverages using a portable microchip capillary electrophoresis system.

摘要: A portable microfabricated capillary electrophoresis (CE) instrument is used for the determination of neurologically active biogenic amines, especially tyramine and histamine, in fermented beverages. The target molecules are labeled on their primary amino groups with fluorescamine a 10-min reaction, samples analyzed directly, producing detailed electropherogram only 120 s glass CE device containing 21.4-cm-long separation channels. Tyramine was found mainly red wines at <1-3.4 mg/L, while histamine content these ranged from 1.8 to 19 mg/L. highest levels (20-40 mg/L) were sake. analysis drawn grape crush through malolactic fermentation four varieties zinfandel revealed that produced during yeast fermentation, respectively. Following 3.3 30 1.0 3.0 This highly sensitive rapid lab-on-a-chip method establishes feasibility monitoring amine potentially other chemically allergenically important our food supply.