GSTU43 gene involved in ALA-regulated redox homeostasis, to maintain coordinated chlorophyll synthesis of tomato at low temperature.

摘要: Exogenous 5-aminolevulinic acid (ALA) positively regulates plants chlorophyll synthesis and protects them against environmental stresses, although the protection mechanism is not fully clear. Here, we explored effects of ALA on in tomato plants, which are sensitive to low temperature. We also examined roles glutathione S-transferase (GSTU43) gene, involved ALA-induced tolerance oxidation stress regulation under alleviated temperature caused obstacle uroporphyrinogen III (UROIII) conversion protoporphyrin IX (Proto IX), enhanced production its precursors, including endogenous ALA, Proto IX, Mg-protoporphyrin (Mg-proto protochlorophyll (Pchl), leaves. However, did regulate at level transcription. Notably, up-regulated GSTU43 gene protein expression increased GST activity. Silencing with virus-induced silencing reduced activities GST, superoxide dismutase, catalase, ascorbate peroxidase, reductase, membrane lipid peroxidation; while fed significant all these antioxidase antioxidant contents, damage. triggered activity encoded by GSTU43, temperature-induced oxidative stress, perhaps assistance ascorbate- and/or a glutathione-regenerating cycles, actively regulated plant redox homeostasis. This latter effect degree peroxidation, was essential for coordinated chlorophyll.