Nucleic acid primers and probes for the mammalian OB gene

摘要: The present invention relates generally to the control of body weight animals including mammals and humans, more particularly materials identified herein as modulators weight, diagnostic therapeutic uses which such may be put. In its broadest aspect, elucidation discovery nucleotide sequences, proteins putatively expressed by nucleotides or degenerate variations thereof, that demonstrate ability participate in mammalian weight. sequences object represent genes corresponding murine human ob gene, have been postulated play a critical role regulation adiposity. Preliminary data, presented herein, suggests polypeptide product gene question functions hormone. further provides nucleic acid molecules for use molecular probes, primers polymerase chain reaction (PCR) amplification, i.e., synthetic natural oligonucleotides. aspects, cloning vector, comprises acids invention; bacterial, insect, expression invention, operatively associated with an sequence. Accordingly, bacterial cell transfected transformed appropriate correspondingly, above mentioned constructs preparation invention. Also provided are antibodies polypeptide. Moreover, method modulating mammal is provided. specific examples, encoding two isoforms both polypeptides