Molecular cloning and differential expression patterns of copper/zinc superoxide dismutase and manganese superoxide dismutase in Hypophthalmichthys molitrix
作者： Zhi-Wei Zhang , Zhong Li , Hong-Wei Liang , Lin Li , Xiang-Zhong Luo
DOI: 10.1016/J.FSI.2010.11.003
关键词:
摘要: Copper/zinc superoxide dismutase (Cu,Zn-SOD) cDNA and manganese (Mn-SOD) were first cloned from silver carp Hypophthalmichthys molitrix using reverse transcription-polymerase chain reaction (RT-PCR) rapid amplification of ends (RACE) method. The open reading frame (ORF) Cu,Zn-SOD is 465 bp encodes a 154 amino acids (aa) protein, whereas the ORF Mn-SOD 675 bp 224 aa protein. Multiple polypeptide sequence alignment showed high identity both (70–87%) (80–96%) with species compared. Both detected in heart, brain, liver, kidney, spleen, muscle, gill blood. expressed throughout embryogenesis, indicating their important roles during embryonic development specially at cleavage stage. Acute hypoxia suppressed expression liver significantly, up-regulated them relatively, that tissue-specific an stress response adapted to hypoxia.
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