The influence of poly(n-butyl acrylate) networks on viability and function of smooth muscle cells and vascular fibroblasts

摘要: BACKGROUND The patency of small-diameter vascular prostheses is limited by several factors such as thrombogenicity, which strongly influenced surface roughness and chemical composition, or a mechanical mismatch between the elastic modulus an artery prosthesis. A confluent layer endothelial cells onto inner could improve hemocompatibility device. Biomaterials with adjustable properties be tailored to values human arteries so that prothesis avoided. It was recently demonstrated co-culture angiogenically stimulated monocytes (aMO2) shows accelerated formation functional cell monolayer on soft hydrophobic poly(n-butyl acrylate) (cPnBA) networks. In addition, compatibility smooth muscle aortic fibroblasts, are other important types vessel wall, essential for prosthesis material must therefore explored. PURPOSE Here we investigated interaction fibroblasts cPnBA04 cPnBA73. MATERIAL AND METHODS Human primary were seeded two cPnBAs different moduli (cPnBA04 - 250 kPa cPnBA73 1100 kPa) over 72 h. live-dead staining (fluorescein diacetate/propium iodide) performed determine morphology viability adherent cells. Furthermore, extracellular matrix components, actin cytoskeleton, cell-material-contacts cytokine profiles analysed. RESULTS Both adhered viable level pro-inflammatory secretion (IFN-γ TNF-α) comparable cultivated control material. release these cytokines higher compared cPnBA04. secreted CONCLUSION study revealed, cPnBA varying not only suitable cultivation cells, but also fibroblasts. Therefore, potential candidate development cardiovascular prostheses.