Biological and MRI characterization of biomimetic ECM scaffolds for cartilage tissue regeneration.

摘要: Osteoarthritis is the most common joint disorder affecting millions of people. Most scaffolds developed for cartilage regeneration fail due to vascularization and matrix mineralization. In this study we present a chondrogenic extracellular (ECM) incorporated collagen/chitosan scaffold (chondrogenic ECM scaffold) potential use in regenerative therapy. Biochemical characterization showed that these possess key pro-chondrogenic components growth factors. MRI mechanical properties diffusion characteristics important tissue regeneration. In vivo implantation with bone marrow derived mesenchymal stem cells (MSCs) triggered differentiation MSCs without need external stimulus. Finally, results from in vivo experiments indicate are stable MR on par native cartilage. Based our results, envision such have great Additionally, validation parameters histology biochemical analysis indicates ability techniques track progress non-invasively in vivo; highlighting translatory technology.