Imaging collagen packing dynamics during mineralization of engineered bone tissue.

摘要: Abstract The structure and organization of the Type I collagen microfibrils during mineral nanoparticle formation appear as key factor for a deeper understanding biomineralization mechanism governing bone tissue physical properties. In this work we investigated dynamics packing ex-vivo mineralization ceramic porous hydroxyapatite implant scaffolds using synchrotron high resolution X-ray phase contrast micro-tomography (XPCμT) scanning micro diffraction (SμXRD). While XPCμT provides direct 3D image fibers network with micrometer spatial resolution, SμXRD allows to probe structural statistical fluctuations fibrils at nanoscale. particular imaged lateral spacing orientation anisotropic growth nanocrystals. Beyond throwing light on regeneration multiscale process, approach can provide important information in characterization health, aging degeneration conditions. Statement Significance BONE grafts are most common transplants after blood transfusions. This makes bone-tissue research pressing scientific social impact. Bone is complex hierarchical structure, where interplay organic inorganic phases different length scale (from micron atomic scale) affect its functionality health. Thus, requires spatial-temporal evolution (i) (ii) scale. We exploited Phase Contrast Tomography Scanning Diffraction order get new insight engineered mechanisms. could open novel routes early detection degenerative conditions tissue.