Cytochrome P-450 in the housefly: induction, substrate specificity and comparison to three rat hepatic isoenzymes.
作者： S. E. Clarke , C. J. Brealey , G. G. Gibson
DOI: 10.3109/00498258909043170
关键词:
摘要: 1. Both phenobarbital and clofibrate pretreatment cause a qualitative change in cytochrome P-450 content housefly (Musca domestica). Based on changes substrate specificity, both inducing agents appear to induction of an isoenzyme (or group isoenzymes) housefly, this response is similar extent effect that described rodents.2. Using Western blot analysis probing with antibodies three rat hepatic isoenzymes (P450 IA1, P450 IIB1 IVA1), common structural epitopes were sought. Despite similarities patterns catalytic function, little evidence for commonality between the was observed.
sci-hub.se 参考文章(12)
G. Gordon Gibson, Terry C. Orton, Paul P. Tamburini, Cytochrome P-450 induction by clofibrate. Purification and properties of a hepatic cytochrome P-450 relatively specific for the 12- and 11-hydroxylation of dodecanoic acid (lauric acid) Biochemical Journal. ,vol. 203, pp. 161- 168 ,(1982) , 10.1042/BJ2030161
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
T. Nash, The colorimetric estimation of formaldehyde by means of the Hantzsch reaction Biochemical Journal. ,vol. 55, pp. 416- 421 ,(1953) , 10.1042/BJ0550416
Y. Mizukami, K. Sogawa, Y. Suwa, M. Muramatsu, Y. Fujii-Kuriyama, Gene structure of a phenobarbital-inducible cytochrome P-450 in rat liver Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 3958- 3962 ,(1983) , 10.1073/PNAS.80.13.3958
M.J.J. Ronis, E. Hodgson, W.C. Dauterman, Characterization of multiple forms of cytochrome P-450 from an insecticide resistant strain of house fly (Musca domestica) Pesticide Biochemistry and Physiology. ,vol. 32, pp. 74- 90 ,(1988) , 10.1016/0048-3575(88)90123-X
Charles W. Fisher, Richard T. Mayer, Partial purification and characterization of phenobarbital‐induced house fly cytochrome P‐450 Archives of Insect Biochemistry and Physiology. ,vol. 1, pp. 127- 138 ,(1983) , 10.1002/ARCH.940010202
DANIEL W. NEBERT, MILTON ADESNIK, MINOR J. COON, RONALD W. ESTABROOK, FRANK J. GONZALEZ, F. PETER GUENGERICH, IRWIN C. GUNSALUS, ERIC F. JOHNSON, BYRON KEMPER, WAYNE LEVIN, IAN R. PHILLIPS, RYO SATO, MICHAEL R. WATERMAN, The P450 gene superfamily: recommended nomenclature. DNA (Mary Ann Liebert, Inc.). ,vol. 6, pp. 1- 11 ,(1987) , 10.1089/DNA.1987.6.1
Richard T. Mayer, John W. Jermyn, M.Danny Burke, Russell A. Prough, Methoxyresorufin as a substrate for the fluorometric assay of insect microsomal O-dealkylases Pesticide Biochemistry and Physiology. ,vol. 7, pp. 349- 354 ,(1977) , 10.1016/0048-3575(77)90038-4
H. Towbin, T. Staehelin, J. Gordon, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 4350- 4354 ,(1979) , 10.1073/PNAS.76.9.4350
W.Neal Burnette, “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A Analytical Biochemistry. ,vol. 112, pp. 195- 203 ,(1981) , 10.1016/0003-2697(81)90281-5