Occurrence of insertion sequences within the genomes and Tn1546-like elements of glycopeptide-resistant enterococci isolated in Brazil, and identification of a novel element, ISEfa5.

摘要: Abstract Insertion sequences (IS) occur widely within the Tn 1546 -like elements responsible for VanA glycopeptide resistance in enterococci from several countries. As such insertions can be used as epidemiological markers and studying horizontal transfer of gene clusters, we investigated distribution IS 6770 , 1542 1216V 1476 1251 26 Enterococcus faecium 21 faecalis Brazil. PCR, using genomic DNA a template, indicated that most isolates contained (97%), (87%) (72%) elements. was also detected, but at higher frequency E. (80%) than (14%). None harboured . Only two 47 had their elements; one possessed between vanS vanH reported United States; another novel element, designated Efa5 located vanX vanY This element found 25 (96%) 11 (52%) In stability studies, no IS-mediated changes were detected vancomycin-resistant (VRE) monitored over months. These results suggest occurrence Brazilian is similar to American isolates, these rarely vanA clusters. patterns carriage did not correlate with PFGE type VRE, prevalence VRE useful marker. However, presence IS-modified elements, which appear rare Brazil, could molecular marker local studies monitor evolution transmission