Citrate substitutes for homocitrate in nitrogenase of a nifV mutant of Klebsiella pneumoniae

摘要: An organic acid extracted from purified dinitrogenase isolated a nifV mutant of Klebsiella pneumoniae has been identified as citric acid. H{sub 2} evolution by the citrate-containing is partially inhibited CO, and some substrates for nitrogenase. The response maximum velocities to changes in pH both wild-type NifV{sup {minus}} was compared. No substantial differences between enzymes were observed, but there are minor differences. Both stable range 4.8-10, enzyme activities dropped dramatically below 6.2.