Erythromycin, carbomycin, and spiramycin inhibit protein synthesis by stimulating the dissociation of peptidyl-tRNA from ribosomes.

摘要: In mutant Escherichia coli with temperature-sensitive peptidyl-tRNA hydrolase (aminoacyl-tRNA hydrolase; EC 3.1.1.29), accumulates at the nonpermissive temperature (40 degrees C), and cells die. These consequences of high were enhanced if first treated erythromycin, carbomycin, or spiramycin doses sufficient to inhibit protein synthesis in wild-type but not kill either permissive (30 C). Since he is inactivated rapidly irreversibly 40 C, accumulation killing result dissociation, stimulated by antibiotics, from ribosomes. The implications these findings for inhibition cell growth are discussed. Certain alternative interpretations shown be inconsistent relevant data. Previous conflicting observations on effects macrolide antibiotics explained terms our observations. We conclude that (and probably all macrolides) have as a primary mechanism action stimulation dissociation ribosomes, during translocation.