Hypovolemic state: involvement of nitric oxide in the aged related alterations of aquaporins-2 abundance in rat kidney.

摘要: Abstract Aim To examine the effect of nitric oxide (NO) on expression and/or localization inner medulla collecting duct aquaporin-2 water channel (AQP2) in young and adult hemorrhaged anesthetized rats. Methods Rats 2 (young) 12 mo (adult) old ( n  = 15) were divided into: Sham animals with without N G -nitro-l-arginine methyl ester (L-NAME) treatment (S L-NAME S); (20% blood loss) (H H). Mean arterial pressure (MAP) was continuously monitored AQP2 inmunolocalization evaluated at 120 min after bleeding. Results blunted hypotension induced by hemorrhage (106 ± 2 mm Hg) (103 ± 4 mm increased bleeding (from 22 to 50 densitometric units) rats 15 30 units). Pretreatment enhanced this effect, being rise lower than (young: 318%, adult: 233%). Electron microscopy showed that labeling withdrawal, number gold particles smaller medulla. effect. Conclusion NOS activity decreases expression/traffick principal cells response is aging.