Relative roles of mitochondrial and peroxisomal fatty acid oxidation in the metabolism of chylomicron remnants in rats and mice as assessed by a stable-isotope breath test.
作者： Ian J Martins , Royce Vermeulen , Trevor G Redgrave
DOI: 10.1016/S0021-9150(99)00359-7
关键词:
摘要: We have developed a stable isotope breath test to trace physiological remnant metabolism. Validity of the depends on injected lipid emulsion mimicking chylomicron (CR) clearance and subsequent metabolism cholesteryl ester (CE). Oxidation CE fatty acids could involve both mitochondrial peroxisomal pathways. In present studies various agents were used inhibit binding remnants, hydrolysis or acid oxidation. Treatment mice with suramin lactoferrin markedly delayed remnants as shown by significantly lower enrichment 13 CO2 in when compared untreated mice. hepatectomized rats remnant-like emulsions, was virtually abolished. chloroquine methyl palmoxirate (an inhibitor oxidation) impaired recovery label breath. Compared fasted overnight, Intralipid gavage decreased consistent competition between endogenous CR particles. These findings show that reliably measures is metabolised © 2000 Elsevier Science Ireland Ltd. All rights reserved.
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