Silver-ion reversed-phase comprehensive two-dimensional liquid chromatography combined with mass spectrometric detection in lipidic food analysis.

摘要: The triacylglycerol (TAG) profiles present in real world lipidic samples are usually quite complex and, as such, monodimensional high-performance liquid chromatographic (HPLC) techniques inadequate when challenged with such matrices. In this respect, the complementary use of silver-ion (Ag) and non-aqueous (NA) reversed-phase (RP) HPLC can be exploited if thorough TAG separations required. investigation reports employment a newly developed comprehensive LC (LC x LC) system, based on different separation mechanisms aforementioned techniques, applied to rice oil sample. approach was successful high number solutes, otherwise unachievable through LC. Furthermore, atmospheric pressure chemical ionization mass spectrometry (APCI-MS), detection provided third analytical dimension boosting identification power method.