Co-regulation of transcription by BRG1 and BRM, two mutually exclusive SWI/SNF ATPase subunits

摘要: Background: SWI/SNF is a large heterogenous multi-subunit chromatin remodeling complex. It consists of multiple sets mutually exclusive components. Understanding how loss one sibling pair affects the occupancy and function remaining complex needed to understand mutations in particular subunit might affect tumor formation. Recently, we showed that members ARID family subunits (ARID1A, ARID1B, ARID2) had transcriptional relationships including both antagonism cooperativity. However, it remains unknown binding genome-wide remainder Results: We addressed this gap by depleting BRG1, two ATPase characterizing changes its BRM. Additionally, used depletion BRM, or combined determine functional relationship between gene expression. show BRG1 leads site-specific gains losses BRM occupancy. These differences are associated with distinct have cooperative antagonistic interactions. Importantly, at genes where antagonise another observe nearly complete rescue expression BRG/BRM double knockdown. Conclusion: This series experiments demonstrates complexes highlights importance considering role following single subunit.