Integrated study of the structural and metabolic degeneration of skin during 4°C storage in nutrient medium

摘要: Abstract Integrated studies of the structural and metabolic degradation skin during 4 °C refrigerated storage in Eagle's minimal essential medium were undertaken. Skin degeneration occurred three phases first 7 weeks refrigeration. Phase I (Days 1–10) was characterized by release cellular debris leakage intracellular enzymes into medium, shrinkage epithelium, vacuolization cell loss vascular bundles papillary dermis. The accelerated a 2-hr incubation at 25 37 prior to storage. ability dermal cells metabolize glucose, aspartic acid, glutamic ornithine, orotic acid carbon dioxide dropped low levels I. II 11–30) period less intense physical decay, but with continued degeneration. By third week, there nearly total recognizable III 31–58) exfoliation large amounts significant integrity. fifth all nuclei epidermis became pyknotic, reticular dermis lost. Previous reports maximum allowable duration 0–8 have ranged from 0.3 185 days; however, our findings suggest that useful limit nutrient is 1 week if physiological function integrity are desired for optimum postgraft performance.