Molecular single cell analysis demonstrates the derivation of a peripheral blood-derived cell line (L1236) from the Hodgkin/Reed-Sternberg cells of a Hodgkin's lymphoma patient

摘要: A novel cell line, L1236, was established from the peripheral blood of a patient with Hodgkin's disease (HD). Two Ig VH and one Vkappa gene rearrangements were amplified by polymerase chain reaction (PCR) DNA this demonstrating its derivation B-cell lineage. To test line for clonal relationship to Hodgkin/Reed-Sternberg (H-RS) cells in patient, single H-RS micromanipulated tissue sections tumor-infiltrated bone marrow specimen that analyzed PCR rearrangements. The same detected also found repeatedly biopsy material. Thus, L1236 is first case HD which could be demonstrated. Furthermore, selective isolation identical V multiple demonstrates these represented population patient.