Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.

摘要: Disruption-deletion cassettes are powerful tools used to study gene function in many organisms, including Saccharomyces cerevisiae. Perhaps the most widely useful of these heterologous dominant drug resistance cassettes, which use antibiotic genes from bacteria and fungi as selectable markers. We have created three new by replacing kanamycin (kan(r)) open reading frame kanMX3 kanMX4 disruption-deletion (Wach et al., 1994) with frames conferring antibiotics hygromycin B (hph), nourseothricin (nat) bialaphos (pat). The pAG25 (natMX4), pAG29 (patMX4), pAG31 (patMX3), pAG32 (hphMX4), pAG34 (hphMX3) pAG35 (natMX3), cloned into pFA6, so all other respects identical pFA6-kanMX3 pFA6-kanMX4. Most techniques kanMX plasmids can also be hph, nat patMX containing plasmids. These unique phenotypes do not affect growth when inserted ho locus. attributes make ideally suited for creating S. cerevisiae strains multiple mutations within a single strain.