NR2F1-AS1/miR-140/HK2 Axis Regulates Hypoxia-Induced Glycolysis and Migration in Hepatocellular Carcinoma.
作者： Xiao Li , Jing Zhang , Zhengcai Liu , Jingyue Yang , Yize Li
DOI: 10.2147/CMAR.S266797
关键词:
摘要: Background Hypoxia is an important feature for the progression of hepatocellular carcinoma (HCC). Long noncoding RNA nuclear receptor subfamily 2 group F member 1 antisense (NR2F1-AS1) dysregulated in HCC. However, role and mechanism N2RF1-AS1 hypoxia-induced glycolysis migration remain unclear. Materials methods Tumor tissues adjacent samples were harvested from 40 HCC patients. cells treated by hypoxia. The levels NR2F1-AS1, microRNA (miR)-140, hexokinase (HK2) examined via quantitative reverse transcription polymerase chain reaction or Western blot. Glycolysis was analyzed glucose uptake, lactate production, adenosine triphosphate (ATP) levels. Cell transwell assay. target association dual-luciferase reporter assay immunoprecipitation. Results NR2F1-AS1 level enhanced cells. High expression indicated poor overall survival. Silence repressed could regulate HK2 modulating miR-140. miR-140 down-regulation up-regulation mitigated influence silence on Conclusion knockdown restrained increasing decreasing HK2.
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