Molecular characterization of the waxy locus in sorghum.
作者： C. L. McIntyre , J. Drenth , N. Gonzalez , R. G. Henzell , D. R. Jordan
DOI: 10.1139/G08-035
关键词:
摘要: A comparison of approximately 4.5 kb nucleotide sequence from the waxy locus (the granule-bound starch synthase I [GBSS I] locus) a line, BTxARG1, and non-waxy QL39, revealed an extremely high level conservation. Among total 24 differences 9 indels, only 2 changes resulted in altered amino acid residues. Protein folding prediction software suggested that one (Glu to His) may result protein structure, which explain apparently inactive GBSS present BTxARG1. This SNP was not found second RTx2907, does produce I, no other SNPs or indels were 4 obtained RTx2907. Using indel, mapped sorghum chromosome SBI-10, is syntenous maize 9; has been this chromosome. The distribution diverse set germplasm there are two broad types alleles, each type comprising several alleles BTxARG1 RTx2907 have evolved allele types. Glu/His polymorphism derived lines potential as perfect marker for source at locus. correctly predicted phenotype 65% germplasm. co-segregated perfectly with populations crosses between identifying heterozygous lines. Thus, these indel markers sequence-based can be used follow breeding programs selected pedigrees.
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