Atomic force and super-resolution microscopy support a role for LapA as a cell-surface biofilm adhesin of Pseudomonas fluorescens
作者： Ivan E. Ivanov , Chelsea D. Boyd , Peter D. Newell , Mary E. Schwartz , Lynne Turnbull
DOI: 10.1016/J.RESMIC.2012.10.001
关键词:
摘要: Pseudomonas fluorescence Pf0-1 requires the large repeat protein LapA for stable surface attachment. This study presents direct evidence that is a cell-surface-localized adhesin. Atomic force microscopy (AFM) revealed significant 2-fold reduction in adhesion mutants lacking on cell compared to wild-type strain. Deletion of lapG, gene encoding periplasmic cysteine protease functions release from surface, resulted increase adhesion. Three-dimensional structured illumination (3D-SIM) presence consistent with its role as an The only visualized cytoplasm mutant ABC transporter responsible translocating surface. Together, these data highlight power combining use AFM and 3D-SIM genetic studies demonstrate LapA, member group RTX-like proteins, cell-surface
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